mesЭкстремальная биомедицинаExtreme Medicine3033-89643033-8972Centre for Strategic Planning of the Federal Medical and Biological Agency10.47183/mes.2023.043mes-115Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕORIGINAL RESEARCHСемейный случай наследуемой хромосомной интеграции ВГЧ-6А с проведением филогенетического анализаFamilial case of inherited human herpesvirus 6A with phylogenetic assessmentГолеваО. В.GolevaO. V.

Санкт-Петербург

Saint Petersburg 

ДаниловЛ. Г.DanilovL. G.

Санкт-Петербург

Saint Petersburg 

КусакинА. В.KusakinA. V.

Санкт-Петербург

Saint Petersburg 

ЭйсмонтЮ. А.EismontYu. A.

Санкт-Петербург

Saint Petersburg 

БабаченкоИ. В.BabachenkoI. V.

Санкт-Петербург

Saint Petersburg 

ТянН. С.TianN. S.

Санкт-Петербург

Saint Petersburg 

ЧухловинА. Б.ChukhlovinA. B.

Санкт-Петербург

Saint Petersburg 

КрыловА. В.KrylovA. V.

Санкт-Петербург

Saint Petersburg 

ГлотовО. С.GlotovO. S.

Санкт-Петербург

Saint Petersburg 

Детский научно-клинический центр инфекционных болезней Федерального медико-биологического агентстваРоссияPediatric Research and Clinical Center for Infectious Diseases of the Federal Medical Biological AgencyRussian FederationСанкт-Петербургский государственный университетРоссияSaint Petersburg State UniversityRussian FederationДетский научно-клинический центр инфекционных болезней Федерального медико-биологического агентства; Университет ИТМОРоссияPediatric Research and Clinical Center for Infectious Diseases of the Federal Medical Biological Agency; ITMO UniversityRussian FederationПервый Санкт-Петербургский государственный медицинский университет имени академика И. П. ПавловаРоссияPavlov First Saint Petersburg State Medical UniversityRussian FederationДетский научно-клинический центр инфекционных болезней Федерального медико-биологического агентства; Научно-исследовательский институт акушерства, гинекологии и репродуктологии имени Д. О. Отта; Университет ИТМОРоссияPediatric Research and Clinical Center for Infectious Diseases of the Federal Medical Biological Agency; Ott Research Institute of Obstetrics and Gynecology; ITMO UniversityRussian Federation202325102024254144151Copyright © Голева О.В., Данилов Л.Г., Кусакин А.В., Эйсмонт Ю.А., Бабаченко И.В., Тян Н.С., Чухловин А.Б., Крылов А.В., Глотов О.С., 20242024Голева О.В., Данилов Л.Г., Кусакин А.В., Эйсмонт Ю.А., Бабаченко И.В., Тян Н.С., Чухловин А.Б., Крылов А.В., Глотов О.С.Goleva O.V., Danilov L.G., Kusakin A.V., Eismont Y.A., Babachenko I.V., Tian N.S., Chukhlovin A.B., Krylov A.V., Glotov O.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.extrememedicine.ru/jour/article/view/115

В статье рассмотрен семейный случай хромосомной интеграции ВГЧ-6А, которая является важной и актуальной темой в области генетики и медицины. Целью исследования было проверить гипотезу о хромосомной интеграции ВГЧ-6А и его вертикальной передаче у пациента с длительным обнаружением вируса во время рекуррентных респираторных заболеваний, а также в бессимптомный период, при отсутствии жалоб на здоровье. Проведено секвенирование геномной ДНК отца пациента, построено филогенетическое дерево путем выравнивания 270 геномных сборок ВГЧ-6А/В из базы данных GenBank. В результате исследования установлен семейный случай передачи хиВГЧ-6А. Показано, что обнаруженный хиВГЧ-6А, наблюдаемый на филогенетическом древе, находится в тесном контакте с двумя другими хромосомно-интегрированными последовательностями ВГЧ-6А, о которых сообщали московские исследователи. Исследование подтвердило хромосомную интеграцию ВГЧ-6А. Дальнейшее точное хромосомное картирование хиВГЧ-6А/В было бы полезно для исключения вероятных соматических заболеваний, связанных с изменением структуры хромосом при интеграции ВГЧ-6, в частности ВГЧ-6А, а также для идентификации участков инсерции, специфичных для различных географических точек.

The paper reports a familial case of HHV-6A chromosomal integration being an important and relevant issue of genetics and medicine. The study was aimed to test the hypothesis of HHV-6A chromosomal integration and vertical transmission in patient with persistent virus detection during recurrent respiratory diseases and the asymptomatic period when there were no health complaints. Sequencing of the patient’s father genome DNA was performed, and a phylogenetic tree was constructed by aligning 270 HHV-6A/B genome assemblies from the GenBank database. As a result, a familial case of ciHHV-6A transmission was identified. It was found that the detected ciHHV-6A observed on the phylogenetic tree was closely related to other two chromosomally integrated HHV-6A sequences reported by Moscow researchers. The study confirmed HHV-6A chromosomal integration. Further precise chromosome mapping of ciHHV-6A would be useful in terms of excluding probable somatic disorders associated with the chromosome structure alteration following HHV-6, particularly HHV-6A, integration, as well as for identification of insertion sites specific for various geographic locations.

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The authors declare that there are no conflicts of interest present.