mesЭкстремальная биомедицинаExtreme Medicine3033-89643033-8972Centre for Strategic Planning of the Federal Medical and Biological Agency10.47183/mes.2024-26-3-30-39mes-22Research ArticleРАДИОБИОЛОГИЯRADIOBIOLOGYОценка депрессивноподобного поведения у мышей после фракционированного гамма-облученияAssessment of depression-like behavior in mice after fractional gamma irradiationhttps://orcid.org/0000-0001-5914-8913ОбвинцеваН. А.ObvintsevaN. A.

Обвинцева Надежда Александровна

Челябинск

Chelyabinsk

n_obvintseva@mail.ruhttps://orcid.org/0000-0001-8293-2730АтаманюкН. И.AtamanyukN. I.

Атаманюк Наталья Игоревна, канд. биол. наук

Челябинск

Chelyabinsk

vita_pulhra@mail.ruhttps://orcid.org/0000-0002-0769-8267ШапошниковаИ. А.ShaposhnikovaI. A.

Шапошникова Ирина Александровна, канд. биол. наук

Челябинск

Chelyabinsk

shaposhnikova@lenta.ruhttps://orcid.org/0000-0003-0929-9531ПеретыкинА. А.PeretykinA. A.

Перетыкин Андрей Анатольевич

Челябинск

Chelyabinsk

peretykin@urcrm.ruhttps://orcid.org/0000-0002-5990-9118ПряхинЕ. А.PryakhinE. A.

Пряхин Евгений Александрович, д-р биол. наук, профессор

Челябинск

Chelyabinsk

pryakhin@urcrm.ruУральский научно-практический центр радиационной медицины Федерального медико-биологического агентстваРоссияUrals Research Center for Radiation Medicine of the Federal Medical Biological AgencyRussian Federation2024221020242633039Copyright © Обвинцева Н.А., Атаманюк Н.И., Шапошникова И.А., Перетыкин А.А., Пряхин Е.А., 20242024Обвинцева Н.А., Атаманюк Н.И., Шапошникова И.А., Перетыкин А.А., Пряхин Е.А.Obvintseva N.A., Atamanyuk N.I., Shaposhnikova I.A., Peretykin A.A., Pryakhin E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.extrememedicine.ru/jour/article/view/22Введение

Введение. Облучение мозга в больших дозах ионизирующего излучения является установленным фактором риска развития новообразований и когнитивных нарушений. Однако нерешенной проблемой остается влияние длительного облучения в малых дозах на головной мозг и высшую нервную деятельность, в том числе на развитие тревожно-депрессивных расстройств.

Цель

Цель. Изучить влияние фракционированного гамма-облучения в дозах 0,1 Гр, 1 Гр и 5 Гр в ранний постнатальный период на показатели депрессивноподобного состояния у мышей линии C57Bl/6 в возрасте 1 и 6 мес.

Материалы и методы

Материалы и методы. Животных облучали в течение первого месяца жизни. Необходимые кумулятивные дозы (0,1 Гр, 1 Гр и 5 Гр) были получены в режиме фракционированного облучения (20 фракций). Были сформированы 2 контрольные группы: интактные животные и ложно облученные животные. Депрессивноподобное состояние оценивали в тесте «удержания хвоста» в возрасте 1 и 6 мес.

Результаты

Результаты. Возрастные изменения проявлялись в снижении депрессивноподобного поведения у мышей в возрасте 6 мес. по сравнению с мышами в возрасте 1 мес. Стресс, вызванный выполнением манипуляций, связанных с облучением, не оказывал существенного влияния на мышей в возрасте 1 мес., но вызывал развитие выраженного депрессивноподобного состояния у этих же животных в возрасте 6 мес. Радиационное воздействие привело к развитию зависимого от дозы антидепрессивноподобного эффекта, который был более выражен у животных в возрасте 6 мес. после фракционированного облучения в дозах 0,1 Гр и выше.

Выводы

Выводы. Фракционированное гамма-облучение в ранний постнатальный период не приводит к развитию депрессивноподобной симптоматики у мышей, а, напротив, характеризуется антидепрессивным действием.

Introduction

Introduction. Exposure of the brain to high doses of ionizing radiation is an established risk factor for the development of neoplasms and associated cognitive disorders. However, the impact of long-term low-dose irradiation on the brain and higher nervous system, including the development of anxiety-depressive disorders, remains an unsolved problem.

Objective

Objective. To study the effect of fractionated gamma-irradiation in doses of 0.1 Gy, 1 Gy and 5 Gy in the early postnatal period on indices of depression-like states in C57Bl/6 mice at the age of 1 and 6 months.

Materials and methods

Materials and methods. The animals were irradiated during the first month of life. Cumulative doses (0.1 Gy, 1 Gy and 5 Gy) were obtained in the mode of fractionated irradiation (20 fractions). 2 control groups were formed comprising intact animals and falsely irradiated animals. The presence of a depression-like state was evaluated in the “tail holding” test at the age of 1 month and 6 months.

Results

Results. Age-related changes were manifested by a decrease in depression-like behavior in 6-month-old mice compared with 1-month-old mice. Stress induced by performing radiation-related manipulations, which had no significant effect on 1-month-old mice, led to the development of marked depression-like states in the same animals at 6 months of age. Radiation exposure led to the development of a dose-dependent antidepressant-like effect, which was more pronounced in animals at the age of 6 months after fractionated irradiation at doses of 0.1 Gy and higher.

Conclusions

Conclusions. Fractionated gamma-irradiation does not lead to the development of depression-like symptomatology in mice in the early postnatal period, but, on the contrary, is characterized by antidepressant action.

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The authors declare that there are no conflicts of interest present.