mesЭкстремальная биомедицинаExtreme Medicine3033-89643033-8972Centre for Strategic Planning of the Federal Medical and Biological Agency10.47183/mes.2025-296mes-296Research ArticleАВИАКОСМИЧЕСКАЯ И МОРСКАЯ МЕДИЦИНАAEROSPACE & MARITIME MEDICINEСравнительная оценка протеомной регуляции состояния костной ткани в 21-суточной антиортостатической гипокинезии (-6°) и 21-суточной «сухой» иммерсииComparative assessment of proteomic regulation of bone tissue during 21-day head-down bed rest (–6°) and 21-day dry immersionhttps://orcid.org/0000-0002-2071-0443ПастушковаЛ. Х.PastushkovaL. Kh.

Пастушкова Людмила Ханифовна - д-р биол. наук

Москва

Ludmila Kh. Pastushkova - Dr. Sci. (Biol.)

Moscow

lpastushkova@mail.ruhttps://orcid.org/0000-0001-9523-5635ГончароваА. Г.GoncharovaA. G.

Гончарова Анна Георгиевна - д-р мед. наук

Москва

Anna G. Goncharova - Dr. Sci. (Med.)

Moscow

goncharova.anna@gmail.comhttps://orcid.org/0000-0002-9646-7275КаширинаД. Н.KashirinaD. N.

Каширина Дарья Николаевна - канд. биол. наук

Москва

Daria N. Kashirina - Cand. Sci. (Biol.)

Moscow

daryakudryavtseva@mail.ruhttps://orcid.org/0000-0001-6783-4200ЛаринаИ. М.LarinaI. M.

Ларина Ирина Михайловна - д-р мед. наук

Москва

Irina M. Larina - Dr. Sci. (Med.)

Moscow

irina.larina@gmail.comИнститут медико-биологических проблем РАНРоссияInstitute of Biomedical Problems of the Russian Academy of SciencesRussian Federation202517112025274558568Copyright © Пастушкова Л.Х., Гончарова А.Г., Каширина Д.Н., Ларина И.М., 20252025Пастушкова Л.Х., Гончарова А.Г., Каширина Д.Н., Ларина И.М.Pastushkova L.K., Goncharova A.G., Kashirina D.N., Larina I.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.extrememedicine.ru/jour/article/view/296Введение

Введение. Экспериментальные возможности во время реального космического полета ограничены, поэтому актуально использование наземных моделей, таких как «сухая» иммерсия (СИ) и антиортостатическая гипокинезия (АНОГ). Изменения костной ткани индуцируются изменением комплекса факторов внешней среды на протеомном уровне, компенсируя изменения, вызванные снижением гравитации и уменьшением двигательной активности, но в дальнейшем с продолжением воздействия включаются другие контуры регуляции.

Цель

Цель. Сравнительная оценка протеомной регуляции состояния костной ткани в 21-суточной антиортостатической гипокинезии (-6°) и 21-суточной «сухой» иммерсии.

Материалы и методы

Материалы и методы. Методами масс-спектрометрии исследовали образцы плазмы крови 8 здоровых испытателей-добровольцев мужчин (средний возраст 20–44 года) в условиях 21-суточной АНОГ и 10 испытателей (средний возраст 23–34 года) в условиях 21-суточной «сухой» иммерсии. Для статистического анализа и определения молекулярных функций и биологических процессов, в которых участвовали белки, применяли программный пакет Perseus. Соответствие основных биологических процессов, согласно генным онтологиям, указанным в базе данных GO, и определенных белков устанавливали с помощью базы знаний системы ANDSystem, STRING.

Результаты

Результаты. Выявлено 9 белков с достоверно изменяющимся уровнем на 21-е сутки АНОГ (р < 0,05) и 8 белков с достоверно изменяющимся уровнем на 21-е сутки СИ (р < 0,05), связанных с биологическими процессами, протекающими в костной ткани. Часть выявленных белков связаны в устойчивые сети белок-белковых взаимодействий, то есть могут коэкспрессироваться. Выделены два общих белка (гаптоглобин и глутатионпероксидаза) на 21-е сутки СИ, 21-е сутки АНОГ.

Выводы

Выводы. Полученные данные впервые обращают внимание на протеомные механизмы регуляции биологических процессов костной ткани у здоровых лиц под влиянием 21-суточной АНОГ и 21-суточной «сухой» иммерсии. Приведены аннотации каждого белка — участника биологических процессов в костной ткани в 21-суточной АНОГ (-6°) и 21-суточной «сухой» иммерсии. Эти результаты имеют большое значение для авиакосмической и клинической медицины.

Introduction

Introduction. Experimental possibilities during actual spaceflight are limited, making ground-based models, such as dry immersion (DI) and head-down bed rest (HDBR) tests, highly relevant. Changes in bone tissue are induced by alterations in a complex set of environmental factors at the proteomic level, compensating for changes caused by reduced gravity and decreased motor activity. However, upon continued exposure, other regulatory circuits are activated.

Objective

Objective. Comparative assessment of proteomic regulation of bone tissue status in 21-day HDBR (tilted at 6°) and 21-day DI tests.

Materials and methods

Materials and methods. Using mass spectrometry methods, plasma samples from 8 healthy male volunteer subjects (mean age 20– 44 years) under the conditions of 21-day HDBR and 10 subjects (mean age 23–34 years) under 21-day DI were studied. The Perseus software was used for statistical analysis and identification of molecular functions and biological processes involving the proteins. The correspondence of major biological processes, according to gene ontologies specified in the GO database, and identified proteins was established using the knowledge base of the ANDSystem and STRING.

Results

Results. Nine proteins with significantly altered levels on Day 21 of HDBR (p < 0.05) and eight proteins with significantly altered levels on Day 21 of DI (p < 0.05) were identified. These proteins are associated with biological processes occurring in bone tissue. Some of the identi© Л.Х. Пастушкова, А.Г. Гончарова, Д.Н. Каширина, И.М. Ларина, 2025 fied proteins form stable protein–protein interaction (PPI) networks, indicating potential co-expression. Two common proteins — haptoglobin (Hp) and glutathione peroxidase (GPx) — were identified on Day 21 of both DI and HDBR.

Conclusions

Conclusions. The findings offer an insight into the proteomic mechanisms regulating biological processes in bone tissue of healthy individuals under the influence of 21-day HDBR and 21-day DI. Annotations for each protein involved in bone tissue biological processes during 21-day HDBR (tilted at 6°) and 21-day DI are provided. These results are of great importance for aerospace and clinical medicine.

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The authors declare that there are no conflicts of interest present.