mesЭкстремальная биомедицинаExtreme Medicine3033-89643033-8972Centre for Strategic Planning of the Federal Medical and Biological Agency10.47183/mes.2023.001mes-85Research ArticleМЕТОДMETHODМодель лечения абсолютного маточного бесплодия на экспериментальных животныхExperimental animal model for treatment of absolute uterine factor infertilityПолстянойА. М.PolstyanoyA. M.

Красноярск

Krasnoyarsk

ПолстянаяО. Ю.PolstyanayaO. Yu.

Красноярск

Krasnoyarsk

РендашкинИ. В.RendashkinI. V.

Красноярск

Krasnoyarsk

ЯкименкоО. Н.YakimenkoO. N.

Красноярск

Krasnoyarsk

ТуценкоК. О.TutsenkoK. O.

Красноярск 

Krasnoyarsk

СадовскийМ. Г.SadovskyM. G.

Красноярск

Krasnoyarsk

ЧерноваА. А.ChernovaA. A.

Анна Александровна Чернова 

ул. Краснодарская 40/4, г. Красноярск, 660133 

Anna A. Chernova 

Krasnodarskaya, 40/4, Krasnoyarsk, 660133 

chernova-krsk@yandex.ruФедеральный Сибирский научно-клинический центр Федерального медико-биологического агентства России ; Красноярский государственный медицинский университет имени В. Ф. Войно-ЯсенецкогоРоссияFederal Siberian Research Clinical Center of the Federal Medical Biological Agency ; Voyno-Yasenetsky Krasnoyarsk State Medical UniversityRussian FederationФедеральный Сибирский научно-клинический центр Федерального медико-биологического агентства РоссииРоссияFederal Siberian Research Clinical Center of the Federal Medical Biological AgencyRussian FederationФедеральный Сибирский научно-клинический центр Федерального медико-биологического агентства России ; Красноярский государственный медицинский университет имени В. Ф. Войно-Ясенецкого ; Федеральный исследовательский центр «Красноярский научный центр СО РАН»РоссияFederal Siberian Research Clinical Center of the Federal Medical Biological Agency ; Voyno-Yasenetsky Krasnoyarsk State Medical University ; Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of SciencesRussian Federation2023241020242513136Copyright © Полстяной А.М., Полстяная О.Ю., Рендашкин И.В., Якименко О.Н., Туценко К.О., Садовский М.Г., Чернова А.А., 20242024Полстяной А.М., Полстяная О.Ю., Рендашкин И.В., Якименко О.Н., Туценко К.О., Садовский М.Г., Чернова А.А.Polstyanoy A.M., Polstyanaya O.Y., Rendashkin I.V., Yakimenko O.N., Tutsenko K.O., Sadovsky M.G., Chernova A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.extrememedicine.ru/jour/article/view/85

До появления современных вспомогательных репродуктивных технологий трансплантацию репродуктивных органов рассматривали как потенциальное лечение яичникового, трубного и маточного бесплодия. Трансплантацию матки можно рассматривать как метод лечения абсолютного маточного бесплодия, подобный пересадке нежизненно важных органов. Однако клиническое применение трансплантации матки у человека вызывает много проблем. Целью исследования было разработать программу по оценке различных хирургических методов реваскуляризации тканей для восстановления репродуктивной функции при маточной форме бесплодия на экспериментальных животных. Выбор кроликов породы шиншилла (n = 20) для экспериментальной работы обусловлен идентичностью строения органокомплекса всех млекопитающих. Для разработки хирургического протокола пересадки тканей репродуктивной системы на лабораторных животных применили инновационную методику по использованию яичниковых артерий вместо маточных, как в стандартном протоколе. Согласно результатам, полученным на лабораторных животных, гемодинамические характеристики кровоснабжения пересаженной матки не меняются, что позволяет имплементировать данную хирургическую методику для использования в эксперименте на трупном материале для пересадки матки. Предлагаемый протокол проведения операции по трансплантации матки обеспечивает высокий уровень приживаемости трансплантанта и его нормальное кровоснабжение с минимизацией рисков травматизации донора и снижением сложности операции.

Reproductive organ transplantation was considered as a potential method for treatment of the ovarian factor, tubal factor, and uterine factor infertility before the advent of advanced assisted reproductive technologies. Uterus transplantation can be considered as the method for treatment of absolute uterine factor infertility similar to transplantation of non-vital organs. However, the clinical use of uterus transplantation in humans causes a lot of problems. The study was aimed to develop a program for assessment of various surgical tissue revascularization techniques for restoration of reproductive function in experimental animals with uterine factor infertility. Chinchilla rabbits (n = 20) were selected for experiments because of the fact that all mammals have similar structure of the organs. The innovative technique involving the use of ovarian arteries instead of uterine arteries (as in the standard protocol) was used in laboratory animals to develop the surgical protocol for transplantation of reproductive tissues. The animal study results show that hemodynamic characteristics of blood supply to the transplanted uterus remain unchanged. This makes it possible to use the surgical method in the experiments on the uterus transplant from a deceased donor. The proposed uterus transplantation protocol ensures high transplant survival rate and normal blood supply to the transplant, along with the reduced risk of injury to the donor and reduced complexity of the surgical procedure.

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The authors declare that there are no conflicts of interest present.